A polymerase chain reaction assay adapted to plum pox potyvirus detection
نویسندگان
چکیده
منابع مشابه
A highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus detection.
A highly sensitive assay, based on polymerase chain reaction amplification of cDNA synthesized from the viral RNA of antibody-captured viral particles, has been developed for plum pox potyvirus (PPV) detection. The reaction, called immunocapture/PCR (IC/PCR), yields a specific 243-bp product. The immunocapture step, by allowing the use of large sample volumes and by the viral particle prepurifi...
متن کاملRapid Detection of Plum Pox Virus from Plant Samples using Polymerase Chain Reaction
Introduction Sharka or plum pox disease is considered one of the most devastating diseases of stone fruits in terms of agricultural impact and economic importance (Dunez and Sutic, 1988; Nemeth, 1994). The disease is detrimental to apricot, peach and plum trees as it results in reduced quality and premature dropping of fruits. The causal agent is plum pox virus (PPV), a member of the Potyvirus ...
متن کاملComparison of monoclonal antibodies and polymerase chain reaction assays for the typing of isolates belonging to the d and m serotypes of plum pox potyvirus.
ABSTRACT Plum pox potyvirus (PPV) isolates may be divided into four groups separated by serological, molecular, and epidemiological differences. Monoclonal antibodies specific for the two major groups of isolates, represented by the D and M serotypes of the virus, have been obtained. Polymerase chain reaction (PCR)-based assays allowing the direct detection and differentiation of PPV isolates h...
متن کاملDevelopment of a Multiplex Polymerase Chain Reaction for Differential Diagnosis of Canary Pox Virus
Background and Aims: A multiplex transcription-polymerase chain reaction (m-PCR) was developed for direct detection and discrimination between canarypox virus (CPV) and other avian poxvirus (APV). Materials and Methods: Three compatible primer sets were designed for m-PCR amplification of different loci fpv126, fpv140, and fpv167 located at highly conserved APV genes. Results: Results showed th...
متن کاملDetection of Listeria monocytogenes with a nonisotopic polymerase chain reaction-coupled ligase chain reaction assay.
A polymerase chain reaction (PCR)-coupled ligase chain reaction (LCR) assay for the specific detection of Listeria monocytogenes (M. Wiedmann, J. Czajka, F. Barany, and C. A. Batt, Appl. Environ. Microbiol. 58:3443-3447, 1992) has been modified for detection of the LCR products with a nonisotopic readout. When a chemiluminescent or a colorimetric substrate for the nonisotopic detection of the L...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 1991
ISSN: 0166-0934
DOI: 10.1016/0166-0934(91)90035-x